Title of article
Proteome Analysis of Mouse Brain Exposed to Chronic Hypoxia
Author/Authors
Gilany، Kambiz نويسنده Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium , , Aerts، Maarten نويسنده Laboratory for Protein Biochemistry and Protein Engineering, Ghent,Belgium , , Dewilde، Sylvia نويسنده Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium , , Devreese، Bart نويسنده Laboratory for Protein Biochemistry and Protein Engineering, Ghent, Belgium , , Moens، Luc نويسنده , , Vafakhah، Mohtaram نويسنده Reproductive Biotechnology Research Center, Avicenna Research Institute, Shahid Beheshti University, Tehran, Iran ,
Issue Information
دوفصلنامه با شماره پیاپی 48 سال 2011
Pages
8
From page
503
To page
510
Abstract
Objective: Chronic hypoxia exists in many diseases, including cancer. The subject of
our study is analysis of molecular pathways affected in the chronically hypoxic mouse brain.
Materials and Methods: Using the emPAI protocol, we performed a quantitative pro- teomic approach to characterize the global proteome in the mouse brain exposed to 7%
for 48 hours.
Results: Utilizing the emPAI protocol to estimate protein abundance and assign molar concentrations to all proteins, we were able to identify 33 proteins with significant changes in their expression.
Conclusion: Deregulated proteins were mainly involved in cell metabolism, apoptosis, Ca2+ signaling, pentose phosphate pathway, 14-3-3 protein mediated signaling cascades and protein degradation. The obtained data will provide some clues for understanding mechanisms with which cells respond and adapt to chronic hypoxia.
Journal title
Cell Journal (Yakhteh)
Serial Year
2011
Journal title
Cell Journal (Yakhteh)
Record number
662130
Link To Document