Genotype Analysis of Hepatitis Delta Virus from Hepatitis B Surface Antigen-Positive Patients Using PCR-RFLP in Tehran, Iran

Background: Hepatitis delta virus is a unique human pathogen responsible for some 20 million infections globally. This virus is dependent on hepatitis B virus for transmission and propagation. Currently, at least three genotypes of hepatitis delta virus with different geographic distribution and clinical manifestations are described. Methods: In this study, hepatitis delta virus RNA of 35 patients’ sera were analyzed by RTsemi- nested polymerase chain reaction. Based on genomic differences of hepatitis delta antigen coding region of hepatitis delta virus RNA among hepatitis delta virus RNA-positive sera, the polymerase chain reaction products were digested with restriction enzymes and studied by restriction fragment length polymorphism. Results: Out of 35 samples, 13 (38.46%) were positive for hepatitis delta virus RNA by RTsemi- nested polymerase chain reaction. All polymorphisms were shown to be genotype I. Out of 13 hepatitis delta virus RNA-positive (13/35), eight were HBeAg negative. Conclusion: Our data indicated that hepatitis delta virus isolates in Tehran are exclusively genotype I.