Insect Growth Regulatory Activity of Adiantum Capillus-veneris Against Plutella Xylostella and Aphis Craccivora in Ethanol and Methanol

Insect growth regulatory activity of compounds derived from Adiantum Capillus-veneris was studied with Ethanol and Methanol fractions against P. xylostella and A. craccivora. Adiantum capillus-veneris was collected from the natural habitats in Palampur (H.P.).The larvicidal activity of Ethanol fraction of Adiantum also showed significant results .In P. xylostella, it showed highest larvicidal activity at higher concentration of 70-85% at 30,000 ppm and at 5,000 ppm conc. the activity was found to be 30-40% between 24-120 h. A.craccivora showed 70-75% activity at 30,000 ppm and 50-70% activity at 5,000 ppm between 24-48 h. The larvicidal activity of Methanol extract also showed the same trend as in Ethanol. It showed highest mortality rate against A. craccivora at higher conc. followed by P. xylostella. In P. xylostella, the activity was 65-85% at 30,000 ppm and 30-40% at 5,000 ppm after 24-120 h. LC50 values were also found to be highest in A. craccivora (9142.2 and 1227.9)in Ethanol, extract after 24 and 48 h as compared to P. xylostella, (8093. 6,778.2, 6777.6, 6367.7 and 5376.9) at 24-120 h respectively. The value of Chi-square tests showed significant results in P. xylostella and non-significant in A. craccivora. The Methanol extract of A.craccivora also showed highest LC50 values i.e, 11135.5, 557.1 and 4257.0 (24, 48 and 72 h).In P. xylostella, LC50 value was recorded to be 10094.8, 9761.2, 7683.8, 6967.2 and 6264.8 respectively. The chi-square tests were found to be non-significant in A.craccivora and significant results in P. xylostella at 24, 48, and 72,120 h and non-significant after 96 h.