Immunomodulatory Effect of CpG Oligodeoxynucleotide on Infectious Bursal Disease Vaccine Antibody Response and Complement Activity in Chicks

The immune stimulatory activities of bacterial DNA are attributed to the presence of a high frequency of an unmethylated CpG dinucleotide motif The aim of the present study was to evaluate the effects of CpG ODN administration on complement activation and humoral immune response as innate and adoptive immune response indexes, respectively. In the present study Chicks were divided into 6 treatment groups; include Normal control, vaccine control, CPG control and 3 different doses of CpG with vaccine. Blood samples were collected at days 7, 14, 21, 28 and 35. IBD vaccine specific antibody and complement activity were evaluated by ELISA and hemolytic technique, respectively. The comparison of antibody titer and complement activity of CpG control and experimental groups with normal control, show significantly (P<0.05, 0.01) higher levels of antibody and complement activity in study groups. According to the present study, it is found that the immunostimulatory effect of CpG ODN can be utilized to create a protective vaccine when given in combination with IBD vaccine plus complement activity. CpG ODN had the ability to augment innate and adoptive immune response in chickens.