Genetic Diversity Analysis among Greengram genotypes using RAPD Markers

Green gram is also one of the important pulse crops. Conventional breeding methods are very much difficult to utilize in the development of new genotypes. Hence incorporation of the molecular approaches along with the conventional techniques is most powerful method. Evaluation of the available wild accessions are more useful for selecting desirable gene sources. Genetic diversity analysis place an important role in this purpose. For this molecular analysis of selected 18 accessions in greengram (representing all nine clusters) was carried out through RAPD markers. Out of ten primers used nine were polymorphic in which the primer OPS-11 exhibited 100 per cent polymorphism. The value of similarity indices 0.72 to 0.91 indicates high genetic similarity among the selected accessions at molecular level.