Biosynthesis of Enzyme Ionic Plasma for Wastewater Treatment using Fruit and Vegetable Waste

Fruit and vegetable waste (FVW) is produced in large quantities in fruit markets and pose many environmental threats due to its high biodegradability. A possible way to dispose of this waste is its anaerobic digestion to biosynthesize enzyme ionic plasma (EIP) to digest FVW. The current study was focused on the biosynthesis of EIP using FVW and optimizing its process variables like substrate ratio, incubation time and temperature. Chopped FVW along with water and brown sugar was added to a plastic container of 2 L capacity under anaerobic conditions. The substrate ratio was optimized using different combinations of FVW and brown sugar at 25°C. The incubation time was varied from 10-20 days. After synthesis, the EIP was filtered for chemical and microbial analysis; microbial count, pH and chemical oxygen demand (COD). Best results were obtained when EIP was prepared from 1 L of water, 0.5 kg of FVW and 0.1 kg of brown sugar. The optimization studies showed best results of EIP biosynthesis after 20 days for 25°C. © 2010 Friends Science Publishers