Determination of Antibiotic Resistance and Plasmid DNA Profiles of Esherichia coli Strains Isolated from Clinical Materials

In this study, E. coli strains, isolated from different clinical materials, were analysed according to their antibiotic resistance patterns and plasmid DNA profiles. When the incidence of E. coli infections are considered, more E. coli infections were observed in females than in males. of the all E. coli strains isolated from clinical materials, 27% were resistant to only one antibiotic, 25% were resistant to 6 antibiotics and resistance to all 8 antibiotics was established as 6%. In addition to these, resistance rate was highest for ampicillin and ciprofloxacin, whereas lowest resistance was displayed against Piperacillin/Tazobactam. According to the antibiotic resistance patterns of E. coli strains, 10 different antibiotypes were generated and the one, which was observed in highest frequency includes ampicillin-resistant property in itself (antibiotype 1). When the plasmid profiles of E. coli strains are examined, the profile which includes single band was observed as the most frequent one. It was also established that most of the E. coli strains, which were classified in that plasmid profile, acquire the properties of antibiotype 1. Besides it was also observed that; some of the E. coli strains, which do not contain any plasmid in their cells showed resistance to various antibiotics. © 2010 Friends Science Publishers