Hairy roots induction from Portulaca oleracea using Agrobacterium rhizogenes to Noradrenaline,s production

ABSTRACT: Noradrenaline is one of the very important secondary metabolite produce by Portulaca oleraceao. Naturally this plant produce small amount of this metabolites so induction of hairy roots can be useful to increase his commercial product. In our research the strains of Agrobacterium rhizogenes were used to induct hairy roots in Portulaca oleracea. To increasing the transduction , different factors as explants (leaf, stem, root), age of explants (14, 28, 56 days), strain of bacteria (15834, C318, 9435, A4), and bacteria concentration (0/2, 0/4, 0/6, 0/8) were used. Inoculation of 14 days explants with 0/6 concentration of AR15834 strain indicated maximum percentage of transduction. PCR analysis with specific primers rolB gene was performed to confirm the transgenic hairy roots production. PCR analysis showed the presence of diagnostic bands with size 780 bp that related to specific reproduction of rolB gene, so the transgenic hairy roots was confirmed. The concentration of Noradrenalin product in the roots were evaluated by HPLC. Our result showed that the concentrate of Noradrenalin in hairy roots (line-A), have been increased 12 fold in comparison with normal roots (Non-HR).