A Novel Method for Determination of Folic Acid by Fast Continuous Cyclic Voltammetry in Pharmaceutical Preparation

In this work, for the first time a fast continuous cyclic voltammetry was used as a highly sensitive detection method for the monitoring of Folic acid in a flow-injection system. It was used for analysis of Folic acid in pure form as well as in its pharmaceutical formulations. The best performance for sensitive monitoring of Folic acid was obtained at pH 2.0, scan rate 70 Vs-1, accumulation potential 300 mV and accumulation time 0.8 s. The most advantages of the proposed detection method are, including, the removal of oxygen from the test solution is not required any more, and detection limit of the method is in sub-nanomolar level. The potential waveform consisting of the potential steps for cleaning, accumulation and potential ramp was continuously applied on an Au disk microelectrode (with a 12.5 ?m in radius). The detection limit of the method for Folic acid was 1.45pg/ml. The relative standard deviation of the method at 2.0×10-7 M was 2.1 % for 10 runs.