Cytotoxic and Apoptogenic Properties of 2-Phenylthia-zole-4- carboxamide Derivatives in Human Carcinoma Cell Lines

Apoptosis, or programmed cell death, is an essential physiological process that plays a critical role in development and tissue homeostasis. Caspases, a family of cysteine-dependent aspartate-directed proteases, play a critical role in the initiation and execution of apoptosis.In this study,cytotoxicity and apoptogenic effect of 2-phenyl 4-carboxamide derivatives were eva-luated in SKNMC(human neuroblastoma), MCF-7(human breast adenocarcinoma) and HT-29(human colon cancer) cell lines. In cell viability was determined by MTT assay. Also, activation of caspase-3 was evaluated by spectrophotometry. The overall cytotoxicity profiles of deriva-tives demonstrated that the HT-29 cell line has more sensitivity respect to other cell lines. Moreo-ver, our observations indicated that 3-F and 2-F derivatives and 4-Cl derivative increased cas-pase-3 activation in three carcinoma cell line compared to control. Collectively, these findings suggest that these derivatives are able to induce apoptosis in cancer cell lines.