Oxidation damage of sulfur dioxide on testicles of mice

The effects of sulfur dioxide (SO2) on levels of thiobarbituric acid reactive substances (TBARS), levels of reduced glutathione (GSH), and the activities of Cu,Zn-superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were investigated in testicles of Kunming albino male mice. SO2 at different concentrations (22, 56, and 112 mg/m3) was administered to animals of SO2 groups in different exposure chambers for 6 h/day for 7 days, while control groups were exposed to filtered air under the same conditions. Our results show that SO2 caused lipid peroxidation and changes in antioxidative status in testicles of mice. Exposure to SO2 at all concentrations tested significantly increased TBARS levels in testicles of mice. SO2 at all concentrations tested tended to decrease activities of SOD and GPx enzymes and levels of GSH relative to control animals, but only the decreases in SOD and GPx activities caused by SO2 exposures of higher concentrations were statistically significant. SO2 at all concentrations tested tended to increase activities of CAT relative to control animals, but the increases of CAT activities caused by SO2 exposures of low concentrations (22 and 56 mg/m3) were statistically significant. These results lead to the conclusion that SO2 exposure can cause oxidative damage to testicles of male mice, and SO2 is a toxin to the reproductive system of mammals, not only to the respiratory system. Further work is required to understand the toxicological role of SO2 in reproduction organs or even sperm from humans and animals.