Expression of the mnpA gene that encodes the mannoprotein of Aspergillus nidulans is dependent on fadA and flbA as well as veA

In this communication, we show that the palB7 mutation drastically reduced the mannose and N-acetylgalactosamine content f the pacA-encoded acid phosphatase secreted by the fungus Aspergillus nidulans at pH 5.0, compared to a control strain. y using mRNA differential display reverse transcription and polymerase chain reaction, we isolated two cDNAs from the ontrol pabaA1 strain that were not detected in the palB7 mutant strain that encode a mannosyl transferase and a NADHbiquinone oxidoreductase. Thus, a defect in the posttranslational mannosylation of proteins could be the consequence of utations in the palB gene, which encodes for a nuclear calpain-like protease that may have specific functions in the processing f transcription factor(s) similar to its homolog, RIM13, in Saccharomyces cereviseae.