Synthesis and incorporation of 2′-amino acid conjugated nucleotides into ribozymes

2′-Amino-2′-deoxyuridine (1) was conjugated with N-Fmoc protected L-amino acids. 5′-O-Dimethoxytritylation followed by 3′-O-phosphitylation afforded 3′-O-phosphoramidites 4a-d. These monomers were incorporated into ribozymes using the solid phase phosphoramidite method. The catalytic activity of these ribozymes is reported.