New non nucleosidic phosphoramidite reagent for solid phase synthesis of biotinylated oligonucleotides

The synthesis of a 4-N-(6-aminohexanoyl)-4-amino-1,3-butanediol backbone functionalized with a protected biotin moiety is described. The CE phosphoramidite building block was used to incorporate the reporter group into a synthetic probe during solid phase synthesis. The ability of the modified oligomers to form DNA duplex structures with their target sequences and the stability of these structures was measured. Finally, the addition of an extra dT at the 5′-end allowed an efficient 5′-end 32P labelling of the biotinylated oligomers.