Kinetics and mechanisms of release of serum proteins of intoxicated rat livers implanted into guinea pigs

We examined the organic chemistry, kinetics, and mechanisms of release of rat serum proteins by aseptic rat donor livers (DL) pathologically intoxicated by CCl4, alloxan, or cholera toxin implanted into the circulatory systems in parallel with the host livers (HL) of guinea pigs (GP). This implant model was biologically functional as hepatocytes of the DL released rat plasma proteins into the hostʹs extracellular compartment (ECC) as monitored in GP serum samples collected during 4 h of the experiment. Furthermore, rat DL hepatocytes retained an in vivo preimplantation pattern of protein biosynthesis acquired in response to a regulatory mechanism most likely involving leukocytic endogenous mediator (LEM), kinins, or platelet activating factor (PAF), and consequently secreted the entire spectrum of acute-phase reaction (APR) serum proteins into the ECC of the host GP. Therefore, the impetus to synthesize and release APR proteins into GP circulation by intoxicated rat liver originated within and was retained by injured rat hepatocytes.