Photoregulation of deacylation rate of acyl trypsin derived from photoresponsive inverse substrate

The acyl trypsin was prepared by use of an inverse substrate, which is comprise of a photoresponsive 4-phenylazobenzoyl moiety. The acyl group in acyl trypsin has been shown to isomerize from trans-form (4t-trypsin) to cis-form (4c-trypsin)/from cis-form to trans-form by irradiation of UV–vis light. The deacylation rate of the cis-form (4c-trypsin) has been shown to be 18.6 times faster than that of the trans-form (4t-trypsin).