Title of article
Construction of saccharide-modified DNAs by DNA polymerase
Author/Authors
Masayuki Matsui، نويسنده , , Yoshitaka Nishiyama، نويسنده , , Shin-ichi Ueji، نويسنده , , Yasuhito Ebara، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2007
Pages
5
From page
456
To page
460
Abstract
Novel deoxyribonucleotide triphosphates bearing maltose or lactose groups were synthesized as substrates for DNA polymerase. The incorporation efficiencies of these modified substrates were investigated in both primer extension reactions and PCR. The stability and conformation of saccharide-modified dsDNAs were assessed by UV absorbance melting experiments and CD analysis. Enzymatic incorporation of saccharide-modified substrates can be used for the efficient production of saccharide-modified DNAs.
Keywords
Glycoconjugate , DNA polymerase , Modified nucleotide
Journal title
Bioorganic & Medicinal Chemistry Letters
Serial Year
2007
Journal title
Bioorganic & Medicinal Chemistry Letters
Record number
797675
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