Title of article
Degradation of DNA by bisulfite treatment
Author/Authors
Kazuo Tanaka، نويسنده , , Akimitsu Okamoto، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2007
Pages
4
From page
1912
To page
1915
Abstract
A significant level of target degradation was caused by bisulfite treatment for methylcytosine-selective hydrolysis. The depyrimidination proceeded via addition of bisulfite to pyrimidines in DNA. The quantification with real-time PCR after conventional bisulfite treatment showed a large decrease in the amount of full-length DNA.
Keywords
DNA , Cytosine , Methylcytosine , Bisulfite , Epigenotyping , Hydrolysis
Journal title
Bioorganic & Medicinal Chemistry Letters
Serial Year
2007
Journal title
Bioorganic & Medicinal Chemistry Letters
Record number
797957
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