Title of article
An effective method for the in situ synthesis of DNA–CPG conjugates using chemical ligation technology as tools for SNP analysis
Author/Authors
Akihiro Ohkubo، نويسنده , , Kunihiko Tanaka، نويسنده , , Haruhiko Taguchi، نويسنده , , Kohji Seio، نويسنده , , Hiroshi Nagasawa، نويسنده , , Toshifumi Tsukahara، نويسنده , , Mitsuo Sekine، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2007
Pages
5
From page
5969
To page
5973
Abstract
In this paper, we report a new method for the SNP analysis by using a chemical ligation (CL) technique on CPG plates with high coupling efficiency. This method showed markedly high match/mismatch discrimination ability. Particularly, replacement of thymidine with 2-thiothymidine in DNA probes used in the CL technology resulted in significant improvement of the base discrimination ability of the thymine base in this system.
Keywords
Gene detection , N-unprotected DNA synthesis , 2-Thiothymidine , Chemical ligation , Base recognition , SNP
Journal title
Bioorganic & Medicinal Chemistry Letters
Serial Year
2007
Journal title
Bioorganic & Medicinal Chemistry Letters
Record number
798731
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