Title of article
Cy3-3-acylcholine: A fluorescent analogue of acetylcholine for single molecule detection
Author/Authors
Kenzo Fujimoto، نويسنده , , Yoshinaga Yoshimura، نويسنده , , Makoto Ihara، نويسنده , , Kazuhiko Matsuda، نويسنده , , Yuko Takeuchi، نويسنده , , Takaaki Aoki، نويسنده , , Toru Ide، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2008
Pages
4
From page
1106
To page
1109
Abstract
We synthesized a novel fluorescent analogue of acetylcholine, Cy3-3-acylcholine. The molecular weight of the products agreed with structural predictions. Discrete intensity changes of fluorescent spots due to a single ligand binding/unbinding to nAChR were visualized by TIRF microscopy. The agonist effect of the Cy3-3-acylcholine on nicotinic acetylcholine receptor (nAChR) was confirmed electrophysiologically. This newly synthesized fluorescent analogue will enable us to conduct more elaborate studies on single channel interaction processes between nAChR and ligands.
Keywords
fluorescence , ion channels , Single molecule studies , Receptors
Journal title
Bioorganic & Medicinal Chemistry Letters
Serial Year
2008
Journal title
Bioorganic & Medicinal Chemistry Letters
Record number
799122
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