Association of a NOS1 promoter repeat with Alzheimerʹs disease

The gene encoding NOS-I (NOS1) displays a complex transcriptional regulation, with nine alternative first exons. Exon 1c and 1f are the most abundant forms in the brain. A functional single nucleotide polymorphism (SNP) in exon 1c and a polymorphism in exon 1f, consisting of a variable number of tandem repeats (VNTR) originating short (S) and long (L) alleles, were studied in 184 patients with Alzheimerʹs disease (AD) and 144 gender- and age-matched controls. No differences were found for the Ex1c G-84A. The Ex1f-VNTR S allele was significantly more common in AD (55% versus 44%, P = 0.009, OR = 1.52) as was the S/S genotype (28% versus 14%, P = 0.008; OR = 2.37). The S allele showed a highly significant interaction with the ApoE var epsilon4 allele (OR: 10.83). Therefore, short alleles of the NOS1 exon 1f-VNTR are likely to be susceptibility factors for AD, and interact with the var epsilon4 allele to markedly increase the AD risk.