a high-throughput screening utilizing intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind hiv-1 tar rna specifically

A 16-residue peptide, including the Tat49–57 sequence was labeled with a fluorescein and a tetramethylrhodamine at its N–and C-terminus, respectively. This double dye-labeled peptide was prepared as a tracer for high-throughput screening utilizing intramolecular fluorescence resonance energy transfer (FRET). The binding of the competitor molecules for HIV-1 TAR RNA were monitored and dissociation constants of those molecule were determined by using this tracer. This novel screening system might be useful to discover the drug for HIV-1 TAR RNA