Antibacterial properties of Anthemis gayana leaves essential oil

ABSTRACT: Plants are a large origin of new bioactive molecules with remedial potentials. Only a small amount of living plants on Earth have been phytochemically investigated. The aim of this study was in vitro studing of antibacterial effects of the leaves essential oil of Anthemis gayana. In this study essential oil was obtained by hydro-distillation using Clevenger type apparatus for 4h. It was tested in vitro against 4 bateria. Several dilution of essential oil (1%, 2%, 5%, 10%, 15%, 20%, 40%, 80%) was prepared. Antibacterial activity was tested by using microdilution MIC determination and agar well diffusion assay. Data were analyzed by ANOVA test in the P-Value < 0.001. The results illustrated that essential oil in dilution 80% has more inhibitor activity and most activity were shown against Escherchia coli and Acinetobacter baumannii. The results of the present project indicate that the Anthemis gayana leaves inhibited bacteria growth but their effectiveness varied. These antibacterial activities are likely due to the presence of the terpenes in the essential oil. Therefore we will be able perform researches with extraction of this plant effective compound for the treatment of infectious disease.