Structural studies of the saccharide part of the cell envelope lipooligosaccharide from Haemophilus influenzae strain galEgalK

The structure of the saccharide part of the lipooligosaccharide from Haemophilus influenzae strain galEgalK has been investigated. On treatment of the lipooligosaccharide with acid under mild conditions, followed by reduction with sodium borohydride and gel permeation chromatography, a main fraction was obtained which was studied by methylation analysis, NMR spectroscopy, and FABMS. The material was heterogeneous and contained two major compounds, A and B, and one minor, C. .