An α-l-fucosidase from Penicillium multicolor as a candidate enzyme for the synthesis of α (1⃗3)-linked fucosyl oligosaccharides by transglycosylation

A new α-l-fucosidase was partially purified from the culture broth of Penicillium multicolor, which was available commercially as a freeze dried powder by the name of Lactase-P™. This enzyme catalysed the transglycosylation of fucose residue of p-nitrophenyl-α-l-fucopyranoside to give α-l-Fuc-(1⃗3)-d-Glc or α-l-Fuc-(1⃗3)-d-GlcNAc regioselectively. This enzyme was more stable in the organic co-solvents than the α-fucosidase from Aspergillus niger, which was also proposed previously by us as an enzyme to produce fucosyl oligosaccharides.