Title of article
A new fluorescent assay for sialyltransferase
Author/Authors
Yasuhiro Kajihara، نويسنده , , Toshimi Kamitani، نويسنده , , Tohru Sakakibara، نويسنده ,
Issue Information
هفته نامه با شماره پیاپی سال 2001
Pages
5
From page
455
To page
459
Abstract
A new fluorescent assay for the sialyltransferase reaction was established. After incubation of the sialyltransferase reaction, the sialyloligosaccharide obtained was treated by acid hydrolysis, and then the NeuAc that was released was labeled with 1,2-diamino-4,5-methylenedioxibenzene. The fluorescent-labeled NeuAc could be estimated by HPLC (excitation: 373 nm; emission: 448 nm) and a Lineweaver–Burk plot could be plotted with the data from this analysis.
Keywords
Sialyltransferase assay , Fluorescent labeling , Sialic acid
Journal title
Carbohydrate Research
Serial Year
2001
Journal title
Carbohydrate Research
Record number
963178
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