Title of article
Isolation of oligosaccharides from a partial-acid hydrolysate of pneumococcal type 3 polysaccharide for use in conjugate vaccines Original Research Article
Author/Authors
Dirk J. Lefeber، نويسنده , , Ricardo Gutiérrez Gallego، نويسنده , , Christian H. Grün، نويسنده , , Daniela Proietti، نويسنده , , Sandro DʹAscenzi، نويسنده , , Paolo Costantino، نويسنده , , Johannis P. Kamerling، نويسنده , , Johannes F.G Vliegenthart، نويسنده ,
Issue Information
دوهفته نامه با شماره پیاپی سال 2002
Pages
7
From page
819
To page
825
Abstract
A series of well-defined oligosaccharide fragments of the capsular polysaccharide of Streptococcus pneumoniae type 3 has been generated. Partial-acid hydrolysis of the capsular polysaccharide, followed by fractionation of the oligosaccharide mixture by Sepharose Q ion-exchange chromatography yielded fragments containing one to seven [→3)-β-d-GlcpA-(1→4)-β-d-Glcp-(1→] repeating units. The isolated fragments were analysed for purity by high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) using an IonPac AS11 column, and their structures were verified by 1H NMR spectroscopy and nano-electrospray mass spectrometry. The oligosaccharides can be used to produce neoglycoprotein vaccines with a defined carbohydrate part.
Keywords
Polysaccharide degradation , HPAEC-PAD , Conjugate vaccines , Streptococcus pneumoniae
Journal title
Carbohydrate Research
Serial Year
2002
Journal title
Carbohydrate Research
Record number
963461
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