Enzymatic synthesis of d-glucosone 6-phosphate (d-arabino-hexos-2-ulose 6-(dihydrogen phosphate)) and NMR analysis of its isomeric forms

d-Glucosone 6-phosphate (d-arabino-hexos-2-ulose 6-(dihydrogen phosphate)) was prepared from d-glucosone (d-arabino-hexos-2-ulose) by enzymatic conversion with hexokinase. The isomeric composition of d-glucosone 6-phosphate in aqueous solution was quantitatively determined by NMR spectroscopy and compared to d-glucosone. The main isomers are the α-anomer (58%) and the β-anomer (28%) of the hydrated pyranose form, and the β-d-fructofuranose form (14%).