Title of article
The structure of the antigenic polysaccharide produced by Eubactrium saburreum T15 Original Research Article
Author/Authors
Naomi Sato، نويسنده , , Futoshi Nakazawa، نويسنده , , Teiichiro Ito، نويسنده , , Tsutomu Hoshino، نويسنده , , Etsuro Hoshino، نويسنده ,
Issue Information
دوهفته نامه با شماره پیاپی سال 2003
Pages
8
From page
923
To page
930
Abstract
The antigenic polysaccharide was obtained from the cell wall of Eubacterium saburreum strain T15 by trypsin digestion followed by gel permeation and ion-exchange chromatography. Its structure was determined using acid hydrolysis, methylation analysis, and 1D and 2D NMR spectroscopy. It contained l-threo-pent-2-ulose (Xul), d-fucose (Fuc), and d-glycero-d-galacto-heptose (Hep) in 2:3:3 ratio. Methylation analysis indicated an octasaccharide repeating-unit containing five branches. The 1H and 13C signals in NMR spectra of the sugar residues were assigned by COSY, HOHAHA, and HMQC 2D experiments, and the sequence of sugar residues in the repeating unit was determined by NOESY and HMBC e
Keywords
d-glycero-d-galacto-Heptopyranose , Eubacterium saburreum , l-threo-Pent-2-ulose (l-xylulose) , d-Fucofuranose , 2D NMR spectroscopy data , Antigenic polysaccharide
Journal title
Carbohydrate Research
Serial Year
2003
Journal title
Carbohydrate Research
Record number
963719
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