Title of article
In vitro sulfation of N-acetyllactosaminide by soluble recombinant human β-Gal-3′-sulfotransferase
Author/Authors
P. Greimel، نويسنده , , Sabrina Jabs، نويسنده , , Stefan Storch، نويسنده , , Slim Cherif، نويسنده , , Koichi Honke، نويسنده , , Thomas Braulke، نويسنده , , Joachim Thiem، نويسنده ,
Issue Information
دوهفته نامه با شماره پیاپی سال 2006
Pages
7
From page
918
To page
924
Abstract
Membrane-bound β-Gal-3′-sulfotransferase (GP3ST) was expressed and used for in vitro sulfation of Tamm–Horsfall glycoprotein. Further, the regioselective transfer of sulfate to an N-acetyllactosamine derivative could be realised with soluble chimeric GP3ST, also in combination with Lac transglycosylation by means of β-galactosidase. Two alternative straightforward chemical syntheses for the target compound could be elaborated.
Keywords
N-Acetyllactosaminide-3?-sulfate , Glycosylation , ?-Gal-3?-sulfotransferase , Tamm–Horsfall glycoprotein , Chemoenzymatic synthesis
Journal title
Carbohydrate Research
Serial Year
2006
Journal title
Carbohydrate Research
Record number
964756
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