Title of article
Isolation and characterization of an immunoenhancing glucan from alkaline extract of an edible mushroom, Lentinus squarrosulus (Mont.) Singer
Author/Authors
Sanjoy Kumar Bhunia، نويسنده , , Biswajit Dey، نويسنده , , Kankan K. Maity، نويسنده , , Sukesh Patra، نويسنده , , Soumitra Mandal، نويسنده , , Swatilekha Maiti، نويسنده , , Tapas K. Maiti، نويسنده , , Samir R. Sikdar، نويسنده , , Syed S. Islam، نويسنده ,
Issue Information
دوهفته نامه با شماره پیاپی سال 2011
Pages
6
From page
2039
To page
2044
Abstract
A water-soluble glucan, isolated from the alkaline extract of the fruit bodies of an edible mushroom, Lentinus squarrosulus (Mont.) Singer was found to consist of (1→3,6)-linked, (1→3)-linked, (1→6)-linked, and terminal β-d-glucopyranosyl moieties in a relative proportion of approximately 1:2:1:1. This polysaccharide showed optimum activation of macrophages as well as splenocytes and thymocytes at 10 μg/mL. Structural investigation was carried out using sugar analysis, methylation analysis, periodate oxidation study, and NMR experiments (1H, 13C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of above-mentioned experiments, the structure of the repeating unit of the polysaccharide was established as:
Keywords
Lentinus squarrosulus (Mont.) Singer , Polysaccharide , structure , NMR spectroscopy , Immuno-activation
Journal title
Carbohydrate Research
Serial Year
2011
Journal title
Carbohydrate Research
Record number
967285
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