Biosensors and multiple mycotoxin analysis

An immunochemical biosensor assay for the detection of multiple mycotoxins in a sample is described. The inhibition assay is designed to measure four different mycotoxins in a single measurement, following extraction, sample clean-up and incubation with an appropriate cocktail of anti-mycotoxin antibodies. The different mycotoxins could be detected simultaneously in relevant concentrations within a time frame of 25 minutes, including the time needed for sensor regeneration. The application of the developed assay on presently available miniature SPR devices allows the development for low-cost instruments, which can be used in field measurements.