Study of interferences by several metabolites from Aspergillus spp. in the detection of aflatoxigenic strains in media added with cyclodextrin

Possible interferences by different fungal metabolites were studied in order to optimise fluorescent response enhancement of aflatoxins by addition of cyclodextrins. A number of 10 culture media traditionally used for mold and yeasts culture and their enumeration were added with two concentrations of a methyl derivative of β-cyclodextrin. Representative five aflatoxigenic and four non-aflatoxigenic strains of Aspergillus flavus group along with 23 other Aspergillus species were cultured at 28 °C. In all cases, green-bluish fluorescence over all colonies of the aflatoxigenic strain was observed under UV light, this fluorescence was never displayed by any of the other cultured strains.