14097

Separation of Biojenic Amines Using Dansyl Cloride Derivatization and Mixed Micellar Liquid Chromatography

19

23

5

Separation of some biogenic amines via RP-HPLC using mixed micellar mobile phase was investigated. The compounds were derivatized before hand by dansyl chloride as a chromophoric reagent. Appropriate conditions for separation, were determined by studying factors such as temperature, type and percentage of organic modifier, concentration of surfactants (SDS and Brij-35) and the pH of the mobile phase. The number of theoretical plates (N), asymmetry factor (B/A) and selectivity factor (a) considered as criteria for finding the appropriate conditions for separation. Appropriate condition, were: 40 °C, 40 m M SDS, 0.5 mM Brij-35 and 10% 1-propanol at pH=5.

1197788