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Biosorption of textile dyes and effluents by Pleurotus florida and Trametes hirsute with evaluation of their laccase activity

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The rate and efficiency of decolorization of dyes like Blue CA, Black B133, and Corazol Violet SR were tested to evaluate white rot fungal strains. Trametes hirsu-ta and Pleurotus florida showed the greatest extent of decolorization on nutrient salt media. Maximum decolorization of 200 mg/l of Blue 133 was obtained by 4 days old incubated Pleurotus florida followed by Trametes hirsuta after 6 days. An attempt was made to improve the decolorization activity of both organisms with different concentrations of glucose 1 and 2% (w/v). The decolorization activity may be due to the laccase enzyme of white rot fungi. The production of this enzyme was estimated using solid state fermentation with rice bran as a substrate. It was found that P. florida exhibited 0.175 U/ml of laccase activity followed 0.126U/ml by T hirsute, respectively. Decolourization was found to be more effective with P. florida in the presence of 2% (w/v) glucose. Crude extract containing the laccase enzyme was isolated and confirmed by SDS PAGE.

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