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Colony formation ability of frozen thawed spermatogonial stem cell from adult mouse

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Background: The basis of spermatogenesis is the spermatogonial stem cells (SSCs). The concentration of SSCs is very small. However, a system that supports the proliferation and maintenance of SSCs in vitro could be used to preserve and expand SSCs numbers as well as increase success in transplantation. It is a new avenue to restore spermatogenesis in azoospermia subjects. Objective: Proliferation and enhancement of frozen-thawed SSCs numbers during in vitro culture. Materials and Methods: Both Sertoli and spermatogonial cells were isolated from adult mouse testes. Frozen-thawed spermatogonial cells were cultured in two groups: simple culture (Experimental 1) and co culture with Sertoli cells (Experimental 2). Also, Fresh cells were considered as control groups: simple culture (controlI) and co culture with Sertoli cells (control 2).Assay of the spermatogonial-cell-derived colonies was carried out at the end of each week. Results: Results indicated that the viability rate of the frozen cells after thawing (68.4± I 0.2%) was influenced by cryopreservation procedure significantly (p ::::0.00 I). In addition, the number of the colonies and their diameters in the co-culture system with fresh cells (25.1±5.2 and 205 .8±50 urn, respectively) were more than other groups and the differences were significant (p

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