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Gadolinium-Hematoporphyrin: new potential MRI contrast agent for detection of breast cancer cell line (MCF-7)

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Gadolinium-porphyrins have been synthesized and are currently being investigated as magnetic resonance imaging (MRI) contrast agents . This study aimed to synthesize Gd-hematoporphyrin and applicate it for in vitro detection of breast cancer cell line (MCF-7). Methods: The naturally occurring porphyrin (hematoporphyrin) was inserted with gadolinium (III) nitrate hexahydrate to yield Gd-H. T1 relaxation times and signal enhancement of the contrast agents were presented, and the results were compared. UV spectrophotometer measured the attachment of Gd to the cell membrane ofMCF-7. Results: Most of gadolinium chloride (GdC13) was found in the washing solution, indicate that it didn יt fixed to the breast cell membranes during incubation. Gd-DTPA showed some uptake into the MCF-7 cell membranes with incubation, however, its uptak e was significantly lower than Gd-H. Conclusion: Good cell memberan uptake of Gd-porphyrin is comparable to controls, indicating selective delivery it to the breast cell line and considerable potency in diagnostic MR imaging for detection of breast cancer.

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