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The Effect of Different Hormones and Incubation Periods on in vitro Proliferation of Pineapple (Ananas comosus L.) Merr cv. Smooth Cayenne) Shoot-Tip Culture

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Seven different hormone treatments, namely 6-benzylaminopurine (BAP) at 2, 3 mg L-1 was applied singly and in combination with Indole Acetic Acid (IAA) at 0.18, 0.8 and 1.8 mg L-1, BAP at 3.3 mg L-1 in combination with IAA at 1.8 and 3.3 mg L-1 and triple combination of BAP at 2.3, IAA at 1.8 and Gibberellic acid (GA3) at 1.0 mg L-1 were tested, over four different incubation periods of 30, 45, 60 and 75 days, for their effect in the proliferation and growth of Smooth cayenne pineapple shoot-tip culture. Combined application of BAP at 3.3 and IAA at 1.8 mg L-1 induced the highest proliferation of 19 shoots/explant and the highest total of 121 and 125 shoots over 4 cycles of multiplication. Raising the IAA to 3.3 mg L-1 resulted in the lowest proliferation and stunted shoots. Incorporation of GA3 improved the shoot length but caused drastic reduction in proliferation. The other treatments showed an intermediate effect.

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