Biochemical changes induced by general anesthesia with romifidine as a premedication,midazolam and ketamine induction and maintenance by infusion in donkeys

The objective of this study was to determine the effects of the general anesthesia on same biochemical changes in donkeys. The anesthesia was induced by intravenous (IV) injection of romifidine 0. 1 mg/kg as a premedication,after 5 minutes induction of general anesthesia by (IV) of mixture midazolam 0. 1 mg/kg and ketamine hydrochloride 2. 2 mg/kg in the same syringe. The maintenance of anesthesia was performed by (IV) infusion of a mixture of the midazolam 0. 065 mg/kg/hr. and ketamine 6. 6 mg/kg/hr. The biochemical parameters changes in serum levels of Aspartate aminotransferase (AST),Alanine aminotransferase (ALT) and Alkaline phosphate (ALP) activity as liver enzymes and serum glucose were estimated in zero,15,30,60,120,240 and 480 minutes. The results revealed significant differences (P 0. 05) in the means of AST (U/L) between zero 199. 6 with 30 min 192. 5 and 60 min 191. 5. No significant differences (P 0. 05) in mean enzyme activity of the ALT and ALP. Serum glucose results were shown no significant differences (P 0. 05) in the (control,15,30 minutes) and (60,120 and 240) respectively and significant differences in between and within 480 minutes. The general anesthesia in this protocol was good and had little effect on the liver function and showed increase in serum blood glucose in donkeys.