The prediction of estrus synchronization in Awassi ewes by vulvar biometry and vaginal cytology

The characteristics of exfoliated vaginal cells and vulvar biometry following estrus synchronization via insertion of vaginal sponges impregnated with 6-Methyl- 17- Acetoxyprogesterone (MAP) were investigated with the aim to predict estrus in ten adult Awassian ewes .The animals were maintained on green and concentrated ration with water ad libitium . All measurements in the ewes were taken before insertion of intravaginal sponges and at 0hrs , 24hrs , 72hrs after removed of intravaginal sponges. Nine out of ten ewes showed estrus behavior in synchronization rate 90%.The results of vaginal cytology showed dominancy of parabasal cells 49.9% before insertion of intravaginal sponges, and dominancy of intermediate cells 62.4% at 0 hour after removed of intravaginal sponges , while at 24hrs and 72 hrs. the dominant cells were nucleated superficial cells and intermediate cells in percentage rate of 44.3% and 41.8% respectively. The results showed that the measurements of vulvar biometry of synchronized ewes during the study period increase in the vertical dimension of vulva significantly (P≤0.05) only after 24hrs of removing of intravaginal sponges .