تحضير دواء مصاحب من الاندوميثاسين وحامض الاسكوربيك ودراسة تاثيره على انزيم اسباراتيت امينو ترانسفيريز المنقى جزئيا من امصال مرضى داء السكر من النوع الثاني

Preparation of Prodrug from Indomethacine and Ascorbic acid and study of It's Effect on Asparatate amino transferase Enzyme Partially Purified from Blood of Diabetic Patients Type two.

The aim of this study is to prepare a new ester prodrug. used for this purpose ascorbic acid vitamin C and indomethacin by esterification reaction, and partial purification of the AST from blood serum with diabetes type II, and we following steps : 1-preparation of indomethacin chloride by reaction of indomethacin with thionyl chloride .esterification reaction of the hydroxy group in vitamin C with Indomethacin chloride to produce ester indomethacin . an‎d Identification of Structural formula using spectroscopic methods (FTIR (. 2-measuring the AST activity in the blood serum of people with diabetes and blood serum of healthy people. The study included 35 patients serum sample for Type II diabetes and (25) of the serum sample healthy. The control group was observed high level of probability (p <0.001) in ASTactivity in persons with type II diabetes compared to what it is in healthy people 3-gel filtration technique using Sephadex column G100 , purifying the AST from diabetes Type II the buffer solution (phosphate buffer) with pH (7.4) for the separation of the enzyme 4-enzyme kinetics AST was studied , where studied the effect of different concentrations of the substrate of L_asparatate, also studied the effect of pH The optimum temperature for the action of the enzyme was 37 ° C and studied the effect of reaction time on the activity of the enzyme. 5-seven different concentrations was preparation of the compound (Prodrug) assay in enzyme activity with and without the prodrug in vitro Assess the . There is a decrease in the activity of AST with increasing the concentration of the prodrug . 6-studied the type of inhibition of the prodrugshow The inhibition of non-competitive type . 6 -study of drug released : used different pH (2-4) and pH (8-10)