Using PCR technique and culture to diagnose bull brucellosis in Karbala province

Present study aimed to isolate brucella from testes of bull, 40 testes samples were collected from Karbala abattoir then withdraw sample from epidedimys after that stained with modified ziehl neelsen, samples were positive for stain were cultured on brucella selective media and tested with PCR. Results showed 8 samples were positive for modified ziehl neelsen(MZN) , 3 samples were positive for culture and 5 samples were positive for PCR. Concluded that PCR is more effective in diagnose bull brucellosis.