A Comparative Study of Efficacy of Tramadol and Diclofenac on Healing of Full-thickness Cutaneous Wounds in Rabbits

The present study was investigate to compare the effects of tramadol and Diclofenac on healing rates of skin wounds in rabbits based on histopathological evaluation. Twenty four adult clinically healthy rabbits from both sexes weighing from (1.1- 1.9 kg) were used in this study. All animals were subjected to creation of full-thickness wound (4cm2) in abdominal region. The treatment schedule was twice daily with injection of normal saline (group A) (control group), tramadol 10mg/kg b.w. (group B) and diclofenac 1.5mg/kg b.w. (group C) intramuscularly each group contain eight rabbits. On day 3, 7, 10 and 14 post wounding skin biopsies were collected for histopathological two rabbits/period examination. The result revealed, Clinically, the rate of wound healing was same in all groups, histopathological result was show: In 3rd day of skin wound healing group (A) show normal epidermis, dermis and stratum spinoseum, group (B) show moderate hyperplasia of stratum spinoseun and group (C) show severe hyperplasia of stratum spinoseum. In 7th day of skin wound healing group (A) show similar findings of day three, group (B) revealed moderate hyperplasia of stratum spinoseun and abundant keratinocytes while group (C) show severe hyperplasia of stratum spinoseum and reduce of stratum corneum. In 10th day of skin wound group of (A) reflected epidermis and dermis with moderate hyperplasia of stratum spinoseum, in contrast group (B) show moderate hyperplasia of stratum spinoseun with increase in stratum corneum and group (C) revealed reduce in stratum spinoseum and stratum corneum. In 14th day the wound of group (A) show abundant amount of keratin and increase of fibroblasts in dermis, while group (B) show reduce of stratum spinoseum’s hyperplasia and in group (C) there were disappearance of stratum spinoseum’s hyperplasia and reduce of stratum corneum.