EVALUATION OF IMMUNE RESPONSE INDUCED BY VACCINATION WITH TWO DIFFERENT TYPES OF GUMBORO DISEASE VACCINES IN BROILERS

THE STUDY INCLUDED TWO AIMS ,FIRST ONE WAS CHECKING OF SERA ANTIBODIES TITERS BY INDIRECT ELISA, INDUCED BY VACCINATION WITH TWO TYPES (VIRULENCY) OF IBD VACCINES- HOT (IBD-L) THE INTERMEDIATE (GUMBO-L ) IN BROILER CHICKS- ROSS AT 8 DAYS OLD, OTHER AIM WAS CHECKING OF HISTOPATHOLOGICAL CHANGES THAT MAY EXIST DUE TO VACCINATION WITH IBD VACCINES.NINTY NINE CHICKS WERE DIVIDED INTO FOUR GROUPS(CONTROL(1),HOT,INTERMEDIATE CONTROL(2)(2 SUBGROUPS TO BE MIXED WITH VACCINATED GROUPS TO DIAGNOSE VIRUS SHEDDING). AT 23 DAYS OLD ,BLOOD SAMPLES WERE COLLECTED . RESULTS INDICATED SIGNIFICANT IMMUNE RESPONSE DUE TO HOT VACCINE IN COMPARE TO THE INTERMEDIATE ONE WHICH FAILED TO INDUCE IMMUNITY,ALSO SERA RESULTS OF SUBGROUPS WERE LIKE THOSE MIXED WITH,MAY BE DUE TO SHEDDING OF VACCINE VIRUS. ALSO THERE WERE NO HISTOPATHOLOGIC CHANGES DUE TO IBD VACCINES.THE RESULTS WERE PARTICULARLY ATTRIBUTED TO THE MATERNAL DERIVED ANTIBODIES LEVELS WHICH INTERFER WITH THE TYPE OF VACCINE USED.