التحري عن البروسيلا ضمن الجبنة باستخدام تقانة التفاعل السلسلي للبوليميراز

Brucellosis is an endemic zoonosis in Syria, affecting large numbers of animals and an increasing number of humans. Brucella is an intracellular pathogen capable of infecting animals and humans. The aim of this study was to develop a polymerase chain reaction (PCR) protocol for the detection of Brucella melitensis in artificially contaminated cheese. In order to determine the detection limits tests were performed by inoculating bovine milk samples with different dilutions of bacteria cells (101 -107 CFU/ml). The assay was able to detect as low as 101 CFU/ml of initial dilution 48 hours after cheese inoculation, including 24 hours of pre-enrichment by using a filter 0.45 µm for DNA extraction protocol. In conclusion, this new technique was faster and more reliable to detect the presence of B. melitensis in artificially contaminated cheese, comparing with conventional microbiological methods.