تنوع ژنتيكي ماهي خياطه Alburnoides eichwaldii در رودخانه آستارا چاي جنوب غرب درياي خزر

Genetic Diversity of Alburnoides eichwaldii, in Astarachi River, Guilan Province

Alburnoides eichwaldii has been reported from in the Sourth Caspian sea basin, Iran. Because of the importance of genetic variation and population structure studies for species conservation and management, the genetic diversity of Alburnoides eichwaldii (30 species) from Astarachi River, Guilan province was investigated using four (BL1-2b CtoF 172 LIeA-071 CnaB-030 ) microsatellite markers. PCR products were electrophoresed on 12% achrylamid gel and stained with silver nitrate. Calculations were done using PowerMarker ver 3.0, PopGene ver 3.2 software. Number of the observed alleles at all loci ranged from 5 to 10 and averaged 8 alleles per locus. Allele sizes at BL1-2b CtoF-172، LIeA-071 CnaB-030 loci were in the range of 123-174, 104-160, 328-392, 123-149bps respectively. Among the four pairs of markers, CnaB-030and CtoF-172 was the lowest and highest polymorphic one. BL1-2b andCnaB-030 loci showed deviation from Hardy-Weinberg equilibrium. The number of effective alleles, expected heterozygosity, observed heterozygosity and polymorphism information content were calculated as 5.15,0.79,0.99 and 0.76 respectively which could show the suitable level of genetic diversity of the stock. Study on other possible stocks of Alburnoides eichwaldii, to provide genetic perspective of this species in Iran would be recommended