Theoretical studies of binding of saccharin with negative charge to p53 protein via molecular docking method

Saccharin, the first artificial sweetener, has no calories, is 300 times sweeter than sugar, and has been used to sweeten various products, including jams,chewing gum, and medications. Saccharin is carcinogenic for the urinary bladder in rats and mice, and most likely is carcinogenic in human beings. The p53 family plays a pivotal role in the regulation of many critical cellular functions and biological process in normal cells. The abnormal expression of p53 contributes to carcinogenesis. Docking studies were performed for saccharin with negative charge , an artificial sweetener with p53 protein involved in cancer by AutoDock 4.2.2. Molecular docking studies of (SA) with negative charge with Human p53 protein exhibited binding interactions. The results showed that the selected ligand showed binding energy -5/93 kcal/mol. Analysis of the molecular docking indicated that SA charged -1 preferentially bound to the p53 protein and led to possible risk of artificial sweeteners to induce cancer seems to be important.