A DNA label free electrochemical nanostructure sensor for Fluorouracil analysis

5-Fluorouracil (5-FU) is a medication which is used in the treatment of cancer. It is a suicide inhibitor and works through irreversible inhibition of thymidylate synthase [1]. It belongs to the family of drugs called the antimetabolites. Determination of this drug is very important in biological and pharmaceutical samples. In this study, a graphene oxide/polypyrrole pencil graphite electrode was fabricated as a high sensitive electrochemical sensor for determination of 5-FU in real samples. The modified electrode was characterized by AFM and electrochemical impedance spectroscopy (EIS) methods. The signals of guanine and adenine oxidation were linear with respect to the 5-FU concentration in the range of 0.3−90 μmol L−1 with a detection limit of 0.09 μmol L−1. The novel sensor was utilized for the determination of 5-FU in real samples.