Cytotoxicity investigation of linear and cyclic carnosine peptide analogues on cancerous MCF-7 cell line

Cytotoxicity investigation of linear and cyclic carnosine peptide analogues on cancerous MCF-7 cell line

Carnosine (β-alanyl-L-histidine) is a dipeptide in the muscle and nervous tissues. In the present perspective, a possible use of linear and cyclic Carnosine analogues as an anti-neoplastic therapeutic, especially for the treatment and their toxicity against MCF-7 using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT assay) were discussed. In this work, synthesized analogues of linear and cyclic Carnosine did not show good toxicity against cell line of MCF-7 using MTT assay. 2-Chlorotritylchloride resin (2-CTC) was used as solid support and a suitable resin. Macrocyclization of linear (N-Trt) Carnosine analogues were done by coupling reagent and then the final deprotection were done on cyclic (N-Trt) Carnosine analogues by treatment of trifluoroacetic acid 95%. The synthesized linear and cyclic Carnosine analogues were characterized by using different methods such as LC-Mass and FT-IR.

Carnosine (β-alanyl-L-histidine) is a dipeptide in the muscle and nervous tissues. In the present perspective, a possible use of linear and cyclic Carnosine analogues as an anti-neoplastic therapeutic, especially for the treatment and their toxicity against MCF-7 using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT assay) were discussed. In this work, synthesized analogues of linear and cyclic Carnosine did not show good toxicity against cell line of MCF-7 using MTT assay. 2-Chlorotritylchloride resin (2-CTC) was used as solid support and a suitable resin. Macrocyclization of linear (N-Trt) Carnosine analogues were done by coupling reagent and then the final deprotection were done on cyclic (N-Trt) Carnosine analogues by treatment of trifluoroacetic acid 95%. The synthesized linear and cyclic Carnosine analogues were characterized by using different methods such as LC-Mass and FT-IR.