Pseudo-chloride peroxidase: Iron-Porphyrin/Cystein/PEG structural view-point

Enzyme mimics are designed mainly for practical applications as artificial enzymes and also for understanding enzymatic mechanisms [1]. The importance of nano-structures on dynamic functions, including catalysts, is now well recognized. In this approach, many attempts have been made to improve and enhance the activity of these pseudo-enzymes by hydrophobic pocket or scaffold. The encapsulation of metallo-porphyrin active-site into polymer scaffold can be classified in colloid chemistry as part of interfaces in solution. The solution consisted of the porphyrin-metal-cysteine complex and polyethylene glycol (PEG) (as a scaffold) has used to model the chloride peroxidase (CLP) by 28% of native enzyme efficiency. Dynamic light scattering indicated that the designed nanozyme has vesicle-like size-structure and zeta potential indicate negative charge of PEG oxygen atoms, laid on external edge of nano-particles. Also TEM images confirmed the multi-cavity vesicular nanozyme.